Summary: AMSBIO has launched CellO-IF, an all-in-one immunofluorescent staining reagent kit that simplifies the labeling of organoids and spheroids in hydrogels or extracellular matrices, preserving delicate structures and cellular integrity.

Takeaways:

  1. Streamlined Process: CellO-IF reduces the traditional labor-intensive, multi-step process of immunofluorescent staining, saving time and effort.
  2. Enhanced Accuracy: The kit maintains the 3D structure of samples, minimizes sample loss, and generates high-quality data quickly.
  3. Improved Visualization: CellO-IF offers enhanced antigen visualization, providing high-resolution images and enabling successful labeling with primary antibodies that were ineffective with traditional methods.

AMSBIO has launched CellO-IF, an all-in-one immunofluorescent staining reagent kit designed to accelerate the labelling organoids and spheroids directly in hydrogels or extracellular matrices, while preserving delicate structures and cellular integrity.

Immunofluorescence (IF) allows detection and localization of antigens in diverse types of tissues in various cell preparations. The technique provides excellent sensitivity and amplification of signal by comparison to immunohistochemistry. However, traditional immunofluorescent staining is a labor intensive, multi-step process. CellO-IF revolutionizes this by streamlining the procedure, according to the company.

Immunofluorescent Staining That Offers Better Accuracy

CellO-IF eliminates the need for harvesting, clearing, transferring, and centrifuging, significantly reducing sample loss, and generating high-quality data within hours. This simplified workflow not only accelerates your research but also delivers 3D preservation of the natural structure of samples, enhancing the accuracy of your results.

Providing enhanced antigen visualization, CellO-IF not only routinely provides stunning high-resolution images but also has been proven to help with labelling samples using primary antibodies that were unsuccessful using traditional methods, according to the company.

Available in Two Optimized Formats

Available in two optimized formats for labelling of 3D and 2D samples, CellO IF is proven to boost the signal of your targets of interest thereby producing the highest quality images and clearer research insights.

Featured Image: Liver cancer spheroid stained using CellO-IF technology. Image: Didem Demirbas, PhD – Jenny Lai, MD-PhD Candidate, Boston Children’s Hospital, Harvard Medical School