The Rapid EZ DNA-Seq library preparation kit from Tecan Genomics, Maennedorf, Switzerland, offers PCR-free generation of sequencing-ready libraries for next-generation sequencing (NGS).
Suited to whole-genome sequencing and metagenomics for samples with varied GC content, as well as amplicon sequencing, the automation-friendly kit complements Tecan’s existing Celero, Rapid, and Ovation Ultralow DNA-Seq library preparation solutions.
The Rapid EZ DNA-Seq library preparation kit is an end-to-end solution for PCR-free generation of NGS libraries in just 2.5 hours. The kit uses Tecan’s robust enzymatic DNA fragmentation and DimerFree library construction technologies, eliminating the need for optimization regardless of input amount. The kit offers a truly ‘add and incubate’ workflow, making it well suited to applications sensitive to PCR bias or PCR artifacts, such as those using microbiome and prokaryotic samples, or sequencing of GC- or AT-rich regions of the human genome.
The kit includes all of the reagents needed for library construction, with preplated unique dual-index adaptors to enable flexible multiplexing and detection of ‘index hopping.’ The simple, flexible workflow can be easily automated on Tecan’s advanced liquid-handling platforms, such as the DreamPrep NGS workstation.
“Our portfolio of innovative DNA-Seq products uses robust chemistries to simplify library preparation, accelerate processing, and improve the quality of sequencing data,” says Rasmus Lind, general manager of Tecan Genomics. “With the development of the Rapid EZ kit, we now have solutions for every sample type—from very low to high-throughput applications.”
For more information, visit Tecan Genomics.