Purigen Biosystems, Pleasanton, Calif, launched the Ionic FFPE Complete Purification Kit. Scientists are now able to consistently recover both DNA and RNA (mRNA and miRNA) simultaneously from formalin-fixed, paraffin-embedded (FFPE) tissue samples in a single workflow. Purigen is showcasing the advantages of the new kit during the virtual Advances in Genome Biology and Technology (AGBT) 2021 annual meeting.
Purigen’s dedicated FFPE to Pure DNA and FFPE to Pure RNA Kits enable scientists to purify and concentrate superior yields of DNA or RNA from precious samples, and the new Ionic FFPE Complete Purification Kit rounds out Purigen’s FFPE purification portfolio. With this addition, Purigen enables users of the Ionic system to simultaneously extract and purify high yields of DNA and RNA from the same FFPE section or scroll—all while using a streamlined workflow that reduces hands-on time by as much as 75% in comparison to manual column or bead-based methods. The combination of a simple workflow with a simultaneous extraction of DNA and RNA at high yield and quality enables researchers to maximize rare or precious FFPE samples.
“Our customers inquire about purifying RNA and DNA from the same FFPE specimens to perform downstream assays,” says Ajay Pandita, CSO at Canopy Biosciences, a Bruker Company. “With the Ionic FFPE Complete Purification Kit, we can simultaneously extract RNA and DNA with less than half of the hands-on time that other methods require.”
The Ionic FFPE Complete Purification Kit utilizes Purigen’s Ionic Purification System, a compact benchtop instrument that enables the automated extraction of pure and abundant nucleic acids from a wide range of sample types, including cultured or sorted cells and FFPE tissue. These biological samples are gently lysed and loaded into an Ionic Fluidic Chip. The Ionic System uses the company’s isotachophoresis (ITP) technology to apply an electric field to the chip, which isolates the nucleic acids in their natural, native form. Since the nucleic acids are not denatured or dehydrated, nor are they bound and stripped from fixed surfaces, the process minimizes fragmentation and eliminates any bead or buffer contamination. The prepared nucleic acids are ready for analysis by downstream techniques such as next-generation sequencing (NGS) or qPCR.
“The FFPE Complete Purification kit takes the advantages of our first kits for FFPE samples a step further by providing a simple workflow to simultaneously purify both RNA and DNA from the same sample,” says Barney Saunders, PhD, CEO of Purigen Biosystems. “Scientists can now access high yields of high-quality DNA and RNA while conserving precious sample and saving time.”
The Ionic FFPE Complete Purification Kit consists of 12 chips and a reagent set with all necessary enzymes and buffers to perform DNA extraction from 48 samples. The kit is available now through Purigen’s direct sales channels. For more information, visit Purigen.
Featured image: The Ionic FFPE Complete Purification Kit from Purigen Biosystems (Courtesy: Purigen)