Bio-Rad Laboratories, Hercules, Calif, launched three StarBright Dyes specifically developed for flow cytometry. StarBright Blue 700, StarBright Violet 440, and StarBright Violet 610 are fluorescent nanoparticles that are conjugated to Bio-Rad’s highly validated flow antibodies, providing maximal brightness and improved resolution. Brighter than other fluorescent dyes with similar excitation and emission spectra, the StarBright dyes provide researchers with an alternative for flow cytometry panels. The brightness of the dyes combined with precise excitation and emission profiles offer improved resolution of rare populations and low-density antigens and easily integrate into multiplex panels.
“StarBright Dyes offer exceptional brightness with specific excitation and emission, allowing researchers to build brighter flow cytometry panels to enable better resolution of positive populations from background without the need for special buffers,” says Mike Blundell, Bio-Rad product manager, Life Science Group. “The unique profile of these dyes combined with Bio-Rad’s high-quality antibodies provides flexibility and offers a reliable alternative for incorporation into flow cytometry protocols.”
Key Benefits of StarBright Dyes:
- Resistant to photobleaching and highly stable with minimal lot-to-lot variation, enabling researchers to achieve consistent and reproducible staining
- Work with all common staining buffers, including special polymer dye staining buffers, for easy integration into multicolor panels
- The unique chemistry ensures no loss of signal in fixation
- Offer narrow excitation and emission profiles for improved resolution in new and existing flow cytometry experiments
StarBright Dyes are compatible with Bio-Rad’s ZE5 Cell Analyzer, S3e Cell Sorter, as well as other conventional and spectral flow cytometers.
For more information about StarBright Blue 700, StarBright Violet 440 and StarBright Violet 610 Dyes, visit Bio-Rad.
Featured image: The StarBright Blue 700, StarBright Violet 440 and StarBright Violet 610 Dyes offer maximal brightness for improved resolution. (Courtesy: Bio-Rad)