Image courtesy of CDC-DPDx

Adept Immunodiagnostics LLC, Philadelphia, now offers the Hemagen Virgo® Chagas IgG ELISA Assay.

The kit is specifically designed for the detection of circulating antibodies to Trypanosoma cruzi, the causative agent of Chagas’ disease (American Trypanosomiasis).

During an initial incubation step, Chagas’ antibodies in patient serum bind specifically to the immobilized antigen and remain in place after a washing step. A second antibody, conjugated to the enzyme horseradish peroxidase (HRP), recognizes human IgG and binds to the patient’s antibodies remaining from the first step.

Following a wash step, in wells where the conjugate remains bound, the enzyme then catalyzes a color change in the substrate (TMB). After the enzymatic reaction is stopped, the result is measured on an EIA Plate reader at 450nm.

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[Source: Adept Immunodiagnostics LLC]