The new MacsXpress Treg isolation kit for humans from Miltenyi Biotec, Bergisch Gladbach, Germany, facilitates fast and efficient isolation of regulatory T (Treg) cells from a maximum of 30 ml of fresh, anticoagulated whole blood. The technique makes it possible to isolate Treg cell populations without using density-gradient centrifugation and with only one labeling step. The isolated cells are immediately available for further analysis or cell cultivation. The kit enables isolation of CD4+, CD25+, and CD127dim/– Treg cells in two steps. In the first step, CD4 cells are removed using immunomagnetic MacsXpress beads, while erythrocytes are simultaneously sedimented. In the second step, magnetic enrichment of CD25+ Treg cells is carried out using a Macs column. The marked Treg cells are held back in the column to be eluted and are then immediately available for cell cultivation or biochemical, physiological, pharmacological, or morphological investigation. For more information, visit Miltenyi Biotec.