Biotech and pharma labs performing endpoint polymerase chain reaction (PCR) can amplify samples in as little as 15 minutes with new Promega GoTaq Rapid Master Mix paired with the Eppendorf Mastercycler X50s thermal cycler. The combination offers one of the fastest endpoint PCR solutions on the market, according to the companies.

“It is not often labs can make a change this simple to increase efficiency and reduce hours in the workday,” says Tom Livelli, vice president, life sciences products & services, Promega Corporation, Madison, Wis. “The Promega and Eppendorf combination speeds time-to-results without sacrificing result quality or accuracy, enabling labs to address high sample volumes and backlogs.”

High-Quality, Fast PCR

The Promega GoTaq Rapid Master Mix is a ready-to-use PCR master mix that includes a Taq polymerase with hot-start antibodies which allow for room-temperature reaction assembly and may reduce primer-dimer and nonspecific DNA amplification. The master mix has been optimized to enable the traditional three-step amplification phases to be combined into two phases, as well as dramatically shortening the time spent in each phase.

Traditional PCR master mixes can take 1 to 2 hours to amplify samples. The GoTaq Rapid Master Mix can decrease that time to 15 minutes or less, depending on instrument and target amplicon size. When paired with an instrument with a rapid temperature ramp rate, like the Eppendorf Mastercycler X50s, amplicons 1kb or greater can be amplified in as little as 15 minutes. In addition to being one of the fastest cyclers on the market, the Mastercycler X50s also offers numerous quality and comfort features. Paired with Promega reagents, this combination delivers rapid and reliable results as well as easy implementation and operation. Moreover, this PCR setup offers seamless expansion options via eco systems or CycleManager X50 software.

Increased Lab Productivity

“Increasing the speed of PCR protocols is beneficial for virtually every PCR user,” says Dr. Philip Müller, PhD, head of workflow solutions at Eppendorf, Hamburg, Germany. “The improved efficiency reduces occupancy for labs with shared thermal cyclers, making the system readily available for the next user. If PCR represents a bottleneck in the workflow, then fast PCR can be an essential improvement in the overall process, ultimately increasing the productivity of the whole lab.”

Featured image: Promega GoTaq Rapid Master Mix paired with Eppendorf Mastercycler X50s thermal cycler is one of the fastest endpoint PCR solutions available. (Credit: Promega)