The Quantidex DNA assay from Asuragen, Austin, Tex, is a multiplexed quantitative polymerase chain reaction (PCR) assay that measures the absolute copy number of PCR-amplifiable DNA in a sample and reports PCR inhibition. The assay determines the functional quality of sample DNA using a quantitative functional index (QFI) score, designating the fraction of total genomic DNA copies that can be successfully amplified. Together, the QFI score and amplifiable copy number provide actionable guidance that informs the input requirements for next-generation sequencing target enrichment and helps ensure a high level of analytical sensitivity and specificity. In addition, the assay flags PCR inhibitors in the sample and provides an opportunity to salvage such samples through a subsequent cleanup step prior to further processing. For more information, visit Asuragen.