Summary: With respiratory co-infections on the rise, 2025 has seen the launch of new multiplex molecular panels that can detect multiple pathogens such as COVID-19, influenza, RSV, and Mycoplasma pneumoniae in a single run. These panels differ from older tests, and lab technicians need to understand their impact on sample preparation, run times, interpretation, workflow, and patient management.


As respiratory infections continue to overlap, clinical laboratories face increasing demand for precise and rapid diagnostics. In response, several manufacturers have developed advanced multiplex molecular panels in 2025, designed to detect co-infections in a single run. These panels target pathogens like COVID-19, influenza, RSV, and Mycoplasma pneumoniae, providing data for patient management and isolation protocols. Understanding how these new panels differ from older multiplex tests and their implications for laboratory operations is essential for clinical laboratory technicians.

Differences from Older Multiplex Tests

The latest multiplex molecular panels offer advancements over earlier iterations. Unlike older models that often focused on a limited number of targets, the new panels can simultaneously detect a broader range of pathogens. This enhancement reduces the need for multiple tests, thus saving time and resources.

Furthermore, the newer panels incorporate improved sensitivity and specificity, minimizing false positives and negatives. This precision is vital for accurate diagnosis and effective treatment planning. Moreover, some of these panels utilize real-time PCR technology, allowing for faster results without compromising accuracy.

These advancements align with the need for comprehensive diagnostics, especially during peak respiratory seasons. As the demand for these tests increases, laboratory technicians must familiarize themselves with the operational nuances these panels introduce.

Sample Preparation, Run Times, and Interpretation

Sample preparation for these new panels remains largely consistent with standard protocols, but technicians should be aware of specific requirements unique to each manufacturer’s kit. Proper training on handling and processing samples will ensure reliable results.

Run times for these panels vary, but many offer a faster turnaround compared to older tests. This reduction in processing time can enhance laboratory efficiency, allowing for quicker decision-making in clinical settings.

Interpreting results from these multiplex panels may present a learning curve for some technicians. The ability to detect multiple pathogens in a single sample requires careful analysis to ensure correct identification and understanding of co-infection dynamics. Training and familiarity with the software and reporting tools provided by manufacturers can aid in accurate interpretation.

Impact on Workflow and Isolation Protocols

The introduction of multiplex molecular panels has implications for laboratory workflow and isolation protocols. With the capability to detect multiple pathogens simultaneously, laboratories can streamline testing processes, reducing the burden on resources and personnel.

Identifying co-infections early allows healthcare providers to implement tailored isolation protocols, minimizing the risk of cross-contamination within healthcare facilities. This proactive approach not only improves patient outcomes but also enhances overall infection control measures.

As awareness of co-infections grows, laboratory technicians play a crucial role in ensuring these advanced diagnostic tools are effectively integrated into clinical practice. Staying informed about regulatory updates, emerging technologies, and best practices in clinical diagnostics will empower technicians to contribute to improved patient care and management decisions.

In conclusion, the rise of multiplex molecular panels marks a significant step forward in respiratory infection diagnostics. By embracing these advancements, laboratory technicians can enhance their expertise and support healthcare providers in delivering precise and timely patient care.