A new study shows that next generation glycan-based rapid diagnostics can be deployed to detect viral infections such as COVID-19. Researchers at the University of Warwick, Iceni Diagnostics Ltd, and University Hospitals Coventry and Warwickshire (UHCW) NHS Trust have demonstrated their technology to detect SARS-COV-2 using sugars rather than antibodies.1

Lateral flow diagnostics (LFDs) have been widely used during the COVID-19 pandemic to provide rapid identification of people with an active infection. These LFDs work by using antibodies, which “stick” to the SARS-COV-2 virus. 

A research team led by Matthew Gibson, PhD, at the University of Warwick has been working with Iceni Diagnostics to develop an alternative system of detection using glycans (sugars), where synthetic polymer chains are used to attach the glycans to the surface of nanoparticles. Viruses commonly use glycans as a “handle” to attach to our cells, and the team mimicked this process to enable detection of SARS-COV-2. 

“This work shows the potential of using glycans as alternative detection reagents, compared to the traditional antibody-based techniques,” says Gibson, professor at Warwick Medical School and the Department of Chemistry at the University of Warwick. “Furthermore, the use of our polymeric linkers, which allows us to present the glycan on the nanoparticles (which make the red line), shows the benefit of true cross-disciplinary, cross-sector collaboration. This work shows that our approach can work with primary clinical samples, and we are actively developing this into a real-world device with our partners.”

Working with UHCW NHS Trust, the team demonstrated that prototype devices could identify COVID-19 positive swabs across a range of viral loads. The team also showed that the technology functioned well with the spike proteins from variants of concern, which is a key benefit of using glycan-binding technology.

“This is a testament to the cutting-edge scientific research taking place at the University of Warwick and UHCW NHS Trust,” says Dimitris Grammatopoulos, PhD, FRCPath, professor at Warwick Medical School and Consultant in Clinical Biochemistry at UHCW NHS Trust. “Initial results of this prototype showed it can perform favorably in comparison to established COVID-19 tests with respect to cost, time, accuracy, and reliability. We are delighted to collaborate on this research.”

This work clearly demonstrates that glycan-recognition technology can be used to identify pathogens, which the academic/industry team are actively developing further as part of a collaborative project.  

“Our ambition was to exemplify how an academic and industry collaboration can translate hard-core scientific discoveries into practical solutions, and this study has proven we can do this by combining our deep experience in glycoscience,” says Rob Field, PhD, Iceni Diagnostics’ chief scientific officer. “The successful testing of the prototype device and glycan-based platform will now enable us to progress our viral and other pathogen pipelines, and we are delighted to continue working with the University of Warwick team on this program.”

Reference

[1] “Glycan-Based Flow-Through Device for the Detection of SARS-COV-2” is published in ACS Sensors, DOI: 10.1021/acssensors.1c01470 Link: https://doi.org/10.1021/acssensors.1c01470