The automation-compatible system works with plasma, serum, urine and cerebrospinal fluid samples from 1 ml to 4 ml input volumes.
New England Biolabs has launched the Monarch Mag Cell-free DNA Extraction Kit, designed to provide scalable extraction of circulating cell-free DNA from biofluids for biomarker discovery and diagnostics development.
The magnetic bead-based workflow is compatible with plasma, serum, urine, and cerebrospinal fluid across variable input volumes from 1 ml to 4 ml. The kit addresses challenges associated with extracting highly fragmented DNA circulating at low concentrations within complex biofluid samples.
“Extracting cell-free DNA is uniquely challenging because it is highly fragmented and circulating at low concentrations within complex and variable biofluid matrices,” says Anagha Kadam, applications and product development scientist II at New England Biolabs, in a release. “The kit utilizes a specialized chemistry and silica-coated magnetic beads to efficiently recover short DNA fragments from challenging samples that inherently exhibit high nuclease activity.”
Designed for Manual and Automated Processing
The kit format offers flexibility across sample number, input amounts, and sample types. Users can manually process individual samples or use automation for higher throughput while maintaining consistent recovery of concentrated cell-free DNA across fragment sizes down to 50 base pairs in length.
The system integrates with New England Biolabs’ existing downstream sequencing and amplification solutions, including the NEBNext portfolio for next-generation sequencing library preparation.
“Bouncing between vendors to troubleshoot an issue is exhausting and inefficient,” says James Deng, senior product marketing manager for New England Biolabs, in a release. “With a cfDNA extraction kit that integrates seamlessly into our downstream sequencing and amplification solutions, we’re empowered to more holistically address customer challenges from sample preparation through molecular diagnostic applications.”
Aligned with the broader Monarch portfolio, the kit reduces plastic usage in reagent bottles and packaging components compared to standard market solutions.
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