The enhanced PCM 2000 personal laser-scanning confocal system includes three-color fluorescence confocal imaging, scanning transmission differential interference contrast (DIC) and new software. The software expands the data handling and display capabilities of the instrument while improving storage and retrieval of images and data files.
Simultaneous three-channel confocal fluorescence is available for recently installed PCM 2000s and as a retrofit on existing systems. The modular system includes two components which can be installed in the field. The first is a three-color excitation module that contains three lasers: Argon ion (488nm); green HeNe (543nm); and red HeNe (633nm). The second component is a simultaneous three-color fluorescence detection module.
At the heart of the detection system is a beam splitter that separates the red and near-infrared emission signals and feeds them to their own individual photomultiplier tubes (PMTs) through optical fiber couplings. The shorter (green) emission signal is directed by fiber to its own PMT.
Another enhancement is the scanning transmission DIC accessory which also can be retrofitted onto existing PCM 2000 systems. It allows for capture of up to two channels of fluorescence images while simultaneously capturing a single scanning transmission image.
In addition, the version 3.5.0 upgrade to Compix’s SimplePCI software is available for the PCM2000. The upgrade features flexibility for input and output TTL triggering, which combined with the Dynamic Intensity software module, provides a data gathering tool for neuroscience and physiology. The dynamic intensity software displays intensity data graphically as it is acquired. Ratiometric fluorescence can be displayed in a spreadsheet or graph that may be saved and exported for publication.
Further software enhancements include one-second acquisition and display of up to three channels of fluorescence image data, which can be displayed separately and merged together on the monitor. Nikon
