Summary:
Thermo Fisher Scientific has launched the One Lambda HybriType HLA Plus Typing Flex kit, an NGS hybrid capture assay designed to enhance HLA research with optimized read balance, flexibility, and high accuracy.
Key Takeaways:
- Flexible Testing – Supports HLA Class I (4 field) and Class II (3 field) genotyping across various sample types.
- High Accuracy & Efficiency – Delivers ≥99.8% concordance with a streamlined workflow requiring less than 5.5 hours.
- Advanced Research Capabilities – Enables deeper investigation into the major histocompatibility complex (MHC) for immunogenetics research.
To enable human leukocyte antigen (HLA) scientists and researchers to continue to advance research of histocompatibility and immunogenetics, Thermo Fisher Scientific has introduced the One Lambda HybriType HLA Plus Typing Flex kit*, a next generation sequencing (NGS) hybrid capture assay featuring a probe-based design that optimizes read balance and uniformity, potentially mitigating allele dropout.
One Lambda HybriType Assay Provides Testing Flexibility
The One Lambda HybriType assay is designed to provide flexibility in testing configuration and coverage, supporting human leukocyte antigen (HLA) Class I (4 field) and Class II (3 field) genotyping analysis across various sample types, including whole blood and buccal swabs.
Its flexible content and extensive gene coverage may minimize ambiguity, delivering results with ≥99.8% concordance. The streamlined workflow, taking less than 5.5 hours with under 2.5 hours of hands-on time, combined with One Lambda TypeStream™ Visual NGS Software, enables rapid automated analysis.
“We developed HybriType to enable our customers to perform deeper research into the major histocompatibility complex (MHC), a genetically rich region that plays a key role in histocompatibility and immunogenetics,” says Tina Liedtky, president, Transplant Diagnostics, Thermo Fisher Scientific. “As our knowledge of the human immune system and how the MHC may impact various conditions continues to evolve, we’re committed to providing our customers with the tools they need to further our collective understanding.”
Key Features Include Simplified Workflow
The One Lambda HybriType assay’s key features include:
- Simple Workflow – Universal protocol for blood and buccals with sample to sequencing in 5.5 hours or less
- Modular Content – 18 loci coverage (includes 11 traditional HLA loci plus 7 additional MHC loci for optional use) plus optional ABO blood group
* For Research Use Only. Not for use in diagnostic procedures.
Featured Image: The One Lambda HybriType HLA Plus Typing Flex kit is a next generation sequencing (NGS) hybrid capture assay featuring a probe-based design that optimizes read balance and uniformity, potentially mitigating allele dropout. Photo: Thermo Fisher Scientific
