Realease releasable antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany, are based on the company’s Realease fluorochrome technology and allow for the one-step removal of antibodies after cell sorting. Sorted cells are label-free, providing optimal flexibility in downstream applications.
Multicolor flow cytometry-based cell sorting is the method of choice for isolating specific cell subsets. However, downstream applications using isolated cells are often limited, since fluorescence channels and epitopes are blocked by the antibody-fluorochrome conjugates utilized for the flow sorting process.
Miltenyi Biotec’s Realease releasable antibodies with the Realease fluorochrome technology can be removed from cells after sorting. The resulting unlabeled cells expand the options for downstream applications. Benefits of Realease releasable antibodies include:
- Allows marker epitopes to become accessible again for downstream applications (eg, for cell culture experiments and cell analysis).
- Permits fluorescence channels to become available again for analysis of relabeled cells (eg, for microscopy applications).
- Enables sequential flow sorting cycles for the isolation of specific cell subsets.
- Provides reproducible results.
For further information, visit Miltenyi Biotec.