Incorporating a novel dual hydrophobically treated matrix filter, Microlute protein precipitation plates (PPPs) from Porvair Sciences, Wrexham, UK, remove interfering proteins from serum, plasma, or whole blood samples prior to analysis by liquid chromatography/mass spectrometry (LC/MS).

Biological samples commonly contain proteins that interfere with downstream applications such as LC/MS analysis. Microlute PPPs use the CRASH method, in which protein is denatured with acetonitrile and the flocculant filtered out, allowing 96 samples to be processed in parallel.

Based on the industry standard MicroLute format, but without the chromatographic sorbent, protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving common problems associated with the CRASH technique of protein clean-up. The dual frit, hydrophobically treated matrix used by the Microlute PPP means that there is no ‘wetting out’ or leakage of sample before the application of vacuum.

For more information, visit Porvair Sciences.