New England Biolabs, Ipswich, Mass, has released the NEBNext library quant kit for Illumina, San Diego. The quantitative polymerase chain reaction (qPCR)-based technology is used for next-generation sequencing library quantitation. Specifically for Illumina sequencing, accurate quantitation enables optimal sequence output by achieving optimal cluster densities. By employing qPCR amplification, it becomes possible to selectively quantitate only those molecules that contain both of the adaptor sequences required for successful sequencing, further increasing the accuracy and specificity of the library quantitative values. With only four required standards, wells are freed up for quantitating additional libraries. The kit includes a concentrated library dilution buffer and ROX, a reference dye used for normalization with some qPCR instruments. A single extension time is required for all libraries, regardless of insert size. For more information, visit New England Biolabs.