Summary:
New England Biolabs showcased advanced sample preparation technologies at AGBT, focusing on improved sequencing data quality and efficiency.

Takeaways:

  1. The NEBNext Enzymatic Methyl-seq v2 kit enables DNA input as low as 100 picograms, broadening research applications.
  2. Streamlined workflows for cell-free DNA and RNA preparation enhance efficiency and data quality.
  3. New epigenetics tools include 5hmC detection, enzymatic DNA fragmentation, and chromatin structure analysis improvements.

New England Biolabs presented a host of technologies aimed at streamlining sample preparation technologies towards the production of high-quality sequencing data at this year’s Advances in Genome Biology and Technology (AGBT) General Meeting.

Specifically, New England Biolabs shared details and experiences around the recently released NEBNext Enzymatic Methyl-seq v2 kit, which expands the dynamic input range for the technology down to 100 picograms of DNA mass, rendering it applicable for a wide range of applications across general and clinical research utilities.

Additionally, presentations will feature streamlined workflows to prepare samples from cell-free DNA and RNA, including Monarch bead-based extraction, through to library preparation using NEBNext Ultra II and UltraExpress DNA and RNA kits. These workflows offer substantial time and efficiency savings, enabling high-quality data from diminishing sample inputs.

New England Biolabs also shared details on the expanded NEBNext portfolio for epigenetics studies, including the adapted Enzymatic 5hmC-seq Kit for detection of 5hmC, as well as new technologies for enzymatic DNA fragmentation, chromatin structure analysis and improvements in data analysis.

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