The kit aims to improve the detection of challenging transcripts, including rare isoforms and long noncoding RNAs.
RNAConnect has commercially launched its UltraMarathonRT cDNA Synthesis and Amplification Kit, designed to enhance short-read RNA sequencing workflows by improving transcript detection and quantification capabilities.
The kit utilizes UltraMarathonRT (uMRT), a group II intron encoded reverse transcriptase that addresses limitations in current RNA sequencing technologies. Unlike traditional retroviral reverse transcriptases that frequently attach and detach from RNA transcripts, uMRT features intrinsic helicase activity that enables ultraprocessive copying of RNA molecules end-to-end in a single pass at ambient temperatures.
“UltraMarathonRT represents a significant leap forward for RNA-seq,” says Dr Jason Underwood, vice president of technology development at RNAConnect, in a release. “By harnessing uMRT, researchers can now uncover RNA species and transcriptional complexity that were previously masked – especially rare isoforms and long noncoding RNAs – unlocking new avenues for biomarker discovery and basic research.”
Addressing cDNA Creation Limitations
Current RNA sequencing workflows face challenges in creating cDNA inputs that accurately reflect transcriptomic diversity in RNA samples. Existing retroviral reverse transcriptases struggle to copy through highly structured, long, or repeat sequences, resulting in RNA stretches that are not converted to cDNA and therefore excluded from sequencing libraries.
The UltraMarathonRT technology aims to solve this problem through its processivity, which allows for more complete transcript coverage. This capability is particularly relevant for detecting challenging RNA species that may be missed by conventional reverse transcription methods.
Performance Claims and Availability
According to RNAConnect, the UltraMarathonRT cDNA Synthesis & Amplification Kit delivers improved performance compared to leading retroviral reverse transcriptase kits, though specific performance metrics were not detailed in the announcement.
The kit is now available worldwide through the company’s website, with flexible sizing options designed to accommodate pilot studies through high-throughput workflows. Custom OEM and bulk packaging options are available for large-scale users and core facilities.
RNAConnect focuses on RNA analysis solutions for genomics, transcriptomics, and molecular diagnostics applications across research areas including rare disease, immunology, oncology, and neurobiology.
Photo caption: RNAConnect’s UltraMarathonRT cDNA Synthesis and Amplification Kit
Photo credit: RNAConnect
