The Repli-g WTA Single Cell Kit from Qiagen, Valencia, Calif, enables whole transcriptome amplification of total RNA or mRNA-enriched (poly A+) from single cells (1 to 1,000 cells). Optimized for use with new technologies, including NGS, the kit is a novel tool for cancer and stem cell research. Dedicated buffers and reagents undergo a controlled decontamination procedure to block amplification of contaminating nucleic acids by the Repli-g method. A lysis buffer stabilizes cellular RNA, ensuring the resulting RNA correctly reflects the in vivo gene expression profile. All enzymatic steps have been developed to enable efficient processing of RNA for accurate amplification of cDNA, which is achieved with negligible sequence bias using innovative multiple displacement amplification (MDA) technology. For more information, visit Qiagen.
November 14, 2017
October 15, 2008