The Callisto Sample Prep System application is optimized for the PromethION platform to support whole genome sequencing workflows.
Volta Labs announced the availability of its DNA Library Preparation Application for the Oxford Nanopore Technologies Ligation Sequencing Kit V14. The new protocol, developed under the Oxford Nanopore Compatible Products Programme, allows laboratories to perform automated, ligation-based library preparation for the PromethION platform.
In benchmarking studies and an independent clinical evaluation at University Medical Center Utrecht, Callisto-prepared libraries reached up to 120 gigabases of output on a single PromethION flow cell. The system delivered diagnostic-grade variant accuracy with 99.66% single nucleotide variant recall.
Clinical Evaluation and Standards
The UMC Utrecht evaluation, presented at the 2026 Advances in Genome Biology and Technology meeting, confirmed that the system’s integrated high molecular weight (HMW) DNA extraction and ligation-based library preparation support routine whole genome sequencing. These workflows are aligned to ISO 15189 standards, according to Volta Labs.
“As we evaluate whole genome sequencing for routine clinical diagnostics, having a robust, automated workflow is essential. Callisto’s HMW DNA extraction and library preparation have given us the consistency and throughput we need to move toward ISO 15189 accreditation for Oxford Nanopore-based sequencing, while delivering variant calling accuracy comparable to our established short-read pipelines,” says Bert van der Zwaag, department of genetics, University Medical Center Utrecht, in a release.
Platform Compatibility
The application is designed to reduce hands-on time for laboratories using the Oxford Nanopore platform. By automating the library preparation process, the system aims to improve consistency for high-throughput sequencing applications.
“The results achieved with libraries prepared with the Callisto Sample Prep System demonstrate the performance our platform can deliver when paired with high-quality sample preparation for whole genome sequencing applications,” says Thomas Bray, vice president, corporate business development at Oxford Nanopore Technologies, in a release.
