Summary: The National Institute of Allergy and Infectious Diseases (NIAID) has awarded grants for 10 projects aimed at developing advanced diagnostic tools for congenital and adult syphilis, addressing the rising syphilis crisis with more precise and efficient testing methods.

Takeaways:

  1. Rising Syphilis Cases: Adult and congenital syphilis cases have surged dramatically, with increases of 80% and 183% respectively between 2018 and 2022, prompting urgent action from the U.S. Department of Health and Human Services.
  2. Need for Advanced Diagnostics: Current syphilis testing relies on outdated methods that struggle to accurately distinguish active infections from past ones, highlighting the need for modern diagnostics that can better guide treatment and assess antibiotic effectiveness.
  3. Innovative Research Funding: NIAID’s $2.4 million in grants will fund research on cutting-edge diagnostic techniques, including molecular methods and point-of-care testing platforms, to improve syphilis detection and management.

The National Institutes of Health’s (NIH) National Institute of Allergy and Infectious Diseases (NIAID) has awarded grants for 10 projects to improve diagnostic tools for congenital and adult syphilis—conditions currently diagnosed with a sequence of tests, each with limited precision. 

Addressing the Syphilis Crisis

The Centers for Disease Control and Prevention (CDC) estimates that adult and congenital syphilis cases increased by 80% and 183% respectively between 2018 and 2022—a crisis that prompted the U.S. Department of Health and Human Services (HHS) to establish a national taskforce to respond to the epidemic.  

“Syphilis antibiotics work, but antiquated testing makes it very difficult to ensure that people are appropriately diagnosed and fully treated,” says NIAID Director Jeanne M. Marrazzo, MD, MPH. “Advanced diagnostics could streamline syphilis care and also enhance our ability to measure the efficacy of candidate syphilis vaccines and other prevention modalities.” 

Syphilis is a common sexually transmitted infection caused by the bacterium Treponema pallidum. It can cause adult neurological and organ damage, as well as congenital abnormalities, stillbirths, and neonatal deaths. The CDC reports that U.S. syphilis incidence has increased since 2000, marked by a sharp rise in cases since 2019 and an escalating toll in medically underserved populations. 

Further reading: Syphilis Cases are Rising, but Many Don’t Know the Symptoms

Improving Syphilis Testing

The current syphilis testing algorithm requires at least two antibody-based tests, which are based on decades-old technology. These tests are unable to reliably distinguish between antibodies from active versus resolved syphilis infection. They also cannot consistently confirm whether a course of antibiotics has successfully cleared the T. pallidum bacteria from the body. Fortunately, modern molecular techniques, such as nucleic acid amplification systems and platforms which detect fragments of a pathogen, are now in use for other infectious diseases and could be adapted for diagnosing syphilis.

The new NIAID grants explore a range of immunologic and diagnostic concepts, including basic research to improve understanding of infant immune responses to syphilis, novel tests to identify different parts of T. pallidum genomic material in infants and adults, measures of antibiotic resistance in T. pallidum strains, and testing platforms that are feasible to use at the point of care rather than an off-site laboratory.

With a cumulative $2.4 million in funding distributed across recipients, the awards are as follows:

  1. Magic Lifescience, Inc., Mountain View, California

Project title: Development of a novel syphilis molecular diagnostic assay for a point-of-care multiplexed genital ulcer panel test on giant magnetoresistive biosensors

Principal investigator: Elaine Ng, PhD (early-stage investigator)

Grant: 1 R21 AI185972-01

  1. Research at Nationwide Children’s, Columbus, Ohio 

Project title: Interrogating infant immune responses for diagnosis of congenital syphilis infection

Principal investigator: Masako Shimamura, MD 

Grant: 1 R21 AI186003-01

  1. University of California San Francisco

Project title: Multi-omic approaches to identify novel biomarkers for the diagnosis of syphilis in pregnancy and assessment of treatment response

Principal investigator: Stephanie Gaw, MD, PhD

Grant: 1 R21AI186006-01

  1. University of Texas at Austin

Project title: A triad approach towards improved diagnostics for maternal and congenital syphilis

Principal investigator: Sanchita Bhadra, PhD (early-stage investigator) with Randolph Hubach, PhD, MPH (Purdue University)

Grant: 1 R21 AI185965-01

  1. University of Texas Southwestern Medical Center, Dallas

Project title: Pre-analytic factors affecting molecular tests for congenital syphilis

Principal investigator: Jeffrey Sorelle, MD, with Emily Adhikari, MD

Grant: 1 R21 AI185968-01

  1. University of Victoria, British Columbia, Canada 

Project title: Development of a direct diagnostic test for infectious and congenital syphilis

Principal investigator: Caroline Cameron, PhD

Grant: 1 R21 AI186005-01

  1. University of Washington, Seattle

Project title: Rapid and ultrasensitive aptamer-based detection technologies for T. pallidum

Principal investigator: Stephen Salipante, MD, PhD

Grant: 1 R21 AI184484-01

  1. University of Washington, Seattle

Project title: Rapid point-of-care detection of T. pallidum resistance to macrolides and tetracyclines by multiplexed loop-mediated amplification (LAMP)

Principal investigator: Joshua Lieberman, MD, PhD (early-stage investigator)

Grant: 1 R21 AI184749-01

  1. University of Washington, Seattle

Project title: Sensitive Treponema pallidum genome recovery through tiling amplicon sequencing

Principal investigator: Alexander Greninger, MD, PhD, MS, M.Phil.

Grant: 1 R21 AI185726-01

  1. Virginia Polytechnic Institute and State University, Blacksburg

Project title: Released peptidoglycan fragments are a biomarker for early stages of syphilis

Principal investigator: Brandon Jutras, PhD

Grant: 1 R21 AI185998-01

Featured image: Electron micrograph imagery of Treponema pallidum, the bacteria that cause syphilis, including a foreground close-up of a single particle (right). Spiral-shaped bacteria are colorized in gold. Photo: NIAID