A new running buffer from Thermo Fisher Scientific, Waltham, Mass, is designed for use at higher voltages than traditional buffers, providing gradient-like electrophoretic separation of protein samples in nearly one-third less time and with a broad molecular weight separation range. The Fisher BioReagents FastRun tris sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) running buffer provides comparable or better resolution and an increased molecular weight separation range of proteins when compared to the traditional tris-glycine-SDS buffer. The buffer system also reduces the number of tris-glycine polyacrylamide gel compositions required to resolve a protein. The buffer can be run at a higher voltage—200 V recommended—because it does not generate excessive heat. The result is a significant improvement in run times. It is compatible with standard tris-glycine polyacrylamide gels, precast or homemade, and with all commercially available protein electrophoresis tanks. For more information, visit Thermo Fisher Scientific.