nRichDX, Irvine, Calif, launched the Semi-Automated Revolution Sample Prep Workflow powered by Eppendorf, Hamburg, Germany, for extraction of cfDNA and ctDNA from a wide range of input sample volumes from 3 mL to 20 mL with market-leading yields and recovery rates of 70% to 90%.
The new semi-automated workflow is designed to increase yield, sensitivity, and throughput by delivering exponentially more target cfDNA and ctDNA for molecular assays with greater automation and reduced hands-on technician time.
The Revolution semi-automated workflow powered by Eppendorf’s epMotion 5073 instrument enables extraction from batches of 8 to 24 samples in as little as 3 hours with 20 minutes of hands-on time.
“nRichDX was founded to advance the preanalytical yield and analytical sensitivity of liquid-based assays,” says nRichDX’s CEO, William Curtis. “The Revolution Semi-Automated Workflow is the next step in nRichDX’s mission to provide unequaled cfDNA yield, higher throughput, and greater operational simplicity for the development of liquid-based assays and the advancement of precision medicine. nRichDX is proud to have worked with leaders in laboratory automation at Eppendorf—a company well known for its lab automation expertise and innovative products—to bring automation to nRichDX’s Revolution Sample Prep System.”
“Analysis of free circulating DNA is a fast-developing application that holds many promises for discovering potential biomarkers in crucial research areas such as cancer research. Using liquid assays allows faster and easier collection of samples,” says Marco Polidori, MBA, PhD, business manager automation for Eppendorf. “At Eppendorf, we are very excited to work together with nRichDx to help customers to automate and standardize processes in the lab. We are convinced that our joint approach provides our customers with an innovative and comprehensive end-to-end solution for their liquid assays.”
The Revolution Semi-Automated Workflow’s current application is for the extraction of cfDNA and ctDNA from plasma and urine for liquid-based assay development, with other applications in the near-term development pipeline for CTCs, exosomes, infectious disease wastewater monitoring, RNA, and total cell-free nucleic acid.
The Revolution Semi-Automated Workflow automates the Revolution protocol’s wash and elution steps, reducing hands-on time and enabling higher sample throughput.
The company is also developing a fully automated instrument expected to be available early next year.
Featured image: The nRichDX Revolution semi-automated workflow powered by Eppendorf’s epMotion instrument enables extraction from batches of 8 to 24 samples in as little as 3 hours with 20 minutes of hands-on time. (Courtesy: nRichDX)