Pharmaceutical companies undertaking absorption, distribution, metabolism, and excretion (ADME) studies are benefiting from integrating Rapid Easy Piercing Seals (EPS) into their screening protocols, according to BioChromato, Fujisawa, Japan.
Optimization of the ADME properties of a drug molecule is one of the most difficult and challenging part of the drug discovery process. The ADME profile of a compound has a major impact on the likelihood of its success as a drug.
Commonly, ADME scientists use 96- or 384-well microplates for storing their large number of samples to be screened. To maintain the integrity of these samples it is important that the microplates be effectively sealed and that the seal not contaminate the samples even after repeated sampling with an HPLC autosampler.
Traditional microplate seals often rely on silicone-based adhesives to create a seal. As a result, sample contamination issues can arise as common HPLC solvents such as acetonitrile, water, and DMSO can extract siloxane out of the silicon based adhesive. By comparison, Rapid EPS seals that use a synthetic rubber adhesive to create a high-integrity, air-tight seal with microplates introduce no contamination into ADME samples to be analyzed by HPLC.
In addition, the unique construction of BioChromato’s Rapid EPS seal leaves no particulate material when pierced by an HPLC Autosampler. This property further safeguards samples from contamination and eliminates damage to, or clogging of, an HPLC autosampler. Rapid EPS seals are proven to offer high-integrity microplate sealing over a working temperature range of -80°C to 80°C.
For further information on Rapid EPS seals, visit BioChromato.
Featured image: Rapid EPS seal provides contamination-free microplate sealing for ADME screening. (Courtesy BioChromato)