Becton, Dickinson and Company (BD), Franklin Lakes, NJ, has released the BD AbSeq assay to analyze protein expression at the single-cell level using high-throughput sequencing.

The assay brings together high-quality antibodies from the BD Pharmingen portfolio with oligonucleotides, enabling researchers to perform single-cell protein analysis on the BD Rhapsody single-cell analysis system. When used together, these tools enable researchers to simultaneously analyze RNA and proteins in thousands of individual cells, and to develop a more complete picture of the role genes and proteins play in biological systems.

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John Ledek, BD Biosciences.

The product leverages 40 years of BD leadership in immunology research. Initially available with more than 100 different human antibody-oligonucleotide combinations, over time BD will expand the portfolio to accommodate its vast library of flow cytometry-proven human and mouse antibody clones, helping researchers to advance understanding of complex diseases. Customers can also design their own custom conjugates.

The launch of the BD AbSeq assay is the latest innovation in BD’s single-cell multiomics portfolio. Over the past year, the company has built on its BD Rhapsody single-cell analysis system—a complete system of reagents, instruments, software, and targeted gene panels—to offer additional single-cell analysis capabilities. These include the BD Rhapsody Express system and the BD single-cell multiplexing kit. These new offerings provide targeted RNA-seq options and enable sample pooling to improve data quality and reduce time to discovery.

“BD is committed to single-cell research and delivering easy-to-use tools with a low barrier to entry,” says John Ledek, worldwide president of BD Biosciences. “Single-cell analysis tools like the BD Rhapsody system and the BD AbSeq assay will maximize our customers’ ability to scrutinize cells of interest and power rapid advancements in immunology, including drug treatment response, cell therapy, and beyond.”

The products described are for research use only and not for diagnostic or therapeutic use. To learn more, visit BD.