Summary: Bio-Rad Labs launched new dye label kits, offering researchers a solution to label antibodies for flexible flow cytometry and fluorescent western blot applications.
Takeaways:
- Enhanced Flexibility: The new kits enable researchers to design custom, high-plex panels for flow cytometry and integrate easily into multiplex fluorescent western blot experiments.
- User-Friendly Protocols: Featuring SpyTag and SpyCatcher technology, the kits streamline antibody labeling with minimal hands-on time, delivering consistent, high-quality results.
- Advanced StarBright Dyes: Bio-Rad’s proprietary fluorescent nanoparticles provide exceptional brightness and compatibility with standard lab equipment, improving detection sensitivity and resolution.
Bio-Rad Laboratories, a provider in life science research and clinical diagnostics products, announced the launch of its TrailBlazer Tag and TrailBlazer StarBright Dye Label Kits. The new kits offer a convenient method to label any antibody with one of Bio-Rad’s StarBright Dyes, for use in either flow cytometry or fluorescent western blot experiments.
The new two-kit system provides researchers across academia, pharma, and biotech access to Bio-Rad’s StarBright Blue and StarBright Violet Dyes, employing Bio-Rad’s SpyTag and SpyCatcher technology to conjugate a dye to an antibody of choice. This enables the design and generation of bespoke, flexible high-plex panels for flow cytometry and easy multiplex integration for fluorescent western blots.
“The new TrailBlazer Tag and StarBright Dye Label Kits are designed for researchers who require greater flexibility in their multiplex experiments, providing multiple label color palette options and access to a select range of our unique StarBright Blue and StarBright Violet Dyes, with additional dyes to be added to the range in the near future,” says Hilary Mavor, marketing director, Life Science Group, Bio-Rad. “With an easy-to-follow protocol and minimal hands-on time, the kits offer a simple process, resulting in user-independent, consistent, high-quality conjugated antibodies.”
Bio-Rad’s range of StarBright Dyes, proprietary fluorescent nanoparticles conjugated to flow-validated antibodies against key immunological targets, offers researchers exceptional brightness with narrow excitation and emission profiles for precise resolution.
Further Reading
StarBright Dyes are compatible with most experimental protocols and flow cytometers, including the Bio-Rad ZE5 Cell Analyzer, without the need for special buffers, providing highly sensitive fluorescent detection, improving resolution of specific cell populations, and minimizing spillover and spreading. For fluorescent western blots, StarBright Blue 700 and 520, designed to work with the configuration of the ChemiDoc MP and ChemiDoc Go Imagers, are labeled to anti-mouse and anti-rabbit secondary antibodies, facilitating bright, highly sensitive fluorescent detection with short exposure times.