The 96-well equilibrium dialyzer for ligand binding experiments includes serum protein binding, protein-drug binding, protein-protein binding, and protein-DNA binding assays.
Each well in the plate consists of two chambers separated by a cellulose acetate membrane with a molecular weight cutoff of 5,000 or 10,000 d. Each chamber holds as many as 250 µL of sample or buffer. During dialysis the plate needs to be rotated 360° in a vertical position. The company supplies a one- or two-plate rotator with a clamp attachment for ease of use. The 96-well plate was designed to meet robotic standards of automation, so it can be automated easily with a standard plate footprint and well spacing.
Harvard Apparatus
(800) 272-2775; www.harvardapparatus.com
