G-Biosciences introduces its XIT™ Mitochondrial DNA kit combining FOCUS™ Mitochondria technology with XIT™ DNA isolation technology in a single kit.
The kit uses our proprietary Subcell buffers to lyse cells and animal tissue in order to remove cellular proteins and nuclei from a highly enriched, intact, and active mitochondrial fraction. The mitochondria are then lysed with the included lysis buffer.
The lysate is treated with our LongLife™ Proteinase K to degrade the proteins. The mitochondrial DNA is then isolated with an alcohol precipitation. The kit is designed for around 100 preparations consisting of 20×106 mammalian cells/ prep. The XIT™ Mitochondrial DNA kit also contains protocols for isolation of mitochondria from soft (i.e. brain & liver) and hard (i.e. cardiac & skeletal muscle) tissue.
Source: G-Biosciences
