photoThe L-Type Fe assay is based on a method that uses bathophenanthroline as a chromogen. When a sample is mixed with buffer, serum protein is denatured by the surfactant contained in the buffer and transferrin-bound iron is liberated.
     The Fe3+ released are reduced to Fe2+ by L-ascorbate and form a chelate with bathophenanthroline disulfonic acid disodium salt. The iron concentration in the sample is determined by measuring the absorbance of the red chelate solution. Iron concentrations are measured spectrophotometrically at 545nm.
     This reagent has no significant interference by ascorbic acid, bilirubin, hemoglobin or intrafat. Within-run precision is 1.62% CV and 1.27% CV at mean concentrations of 60.1µg/dL and 124.5µg/dL, respectively.
     Total precision is 3.69% CV and 3.19% CV at mean concentrations of 64.2µg/dL and 121.8µg/dL, respectively. The minimum detectable limit is 2µg/dL with a linearity limit of 1,000µg/dL.
keyword: test kit, reagent, Fe