The Retrievit Target Retrieval Solutions dramatically enhance immunohistochemistry (IHC) and in situ hybridization (ISH) signals.
False negative and weak staining caused by formalin fixation and tissue processing can now be reversed. The patented microwave pretreatment procedure involves heating tissue sections in a Target Retrieval Solution for about 15 minutes and then letting them cool for 20 minutes prior to immunostaining. The solutions contain an organic buffer, a strong ion chelator and a mild chaotropic reagent that synergistically produce consistently strong signal enhancement. They expand the range of antibodies used for IHC and improve ISH staining results.
Five Retrievit solutions are offered with different pHs for optimizing the staining signal-to-noise ratios for different antigen-antibody pairs.
Keyword: solution, retrieval, IHC, ISH