Thermo Fisher Scientific Inc, Carlsbad, Calif, has launched Invitrogen TaqMan and SYBR Green Fast Advanced cells-to-Ct kits for research use only, strengthening its line of RNA cells-to-Ct products. SYBR Green Fast Advanced cells-to-Ct kits enable faster reverse transcriptase (RT) reactions and access to a quantitative polymerase chain reaction (qPCR) master mix system fully optimized to improve sensitivity for detecting rare transcripts. This makes it possible to perform high-throughput expression analysis directly from cultured cells without RNA purification and without risking sensitivity.
“We are committed to accelerating sample-to-answer for our customers performing gene expression studies and are excited that we can now offer customers an even faster, more-sensitive workflow to measure relative gene expression through real-time RT-PCR without having to purify RNA prior to amplification,” says Ole Dahlberg, vice president and general manager for sample preparation at Thermo Fisher Scientific. “The latest offering in our portfolio of RNA cells-to-Ct kits offers researchers the fastest workflows to date, and higher sensitivity and detection of abundant and rare transcripts.
“These kits help save time and offer a simple workflow that is suitable for a few samples, but can also be easily incorporated into automated, high-throughput applications,” adds Dahlberg. “Researchers can now focus on higher value activities—such as analyzing high-throughput drug screening data or performing supplementary genome editing or silencing validation studies.”
As with previous kits in the product line, the SYBR Green Fast Advanced cells-to-Ct kits employ a 5-minute lysis system to quickly lyse cultured cells while simultaneously removing genomic DNA and preserving RNA integrity. The proprietary cells-to-Ct stop solution irreversibly terminates the lysis reaction, enabling users to carry the most sample into the RT and qPCR reactions to maximize the detection of RNA transcripts. Rare RNA transcript detection in low-input cell samples (10–1,000) is higher with samples processed using cells-to-Ct kits compared with detections using the same samples and RNA purification workflows. The new kits are fully optimized with updated RT reagents for complementary DNA synthesis and faster TaqMan or SYBR Green master mixes for real-time PCR analysis.
To learn more, visit Thermo Fisher Scientific.