Life-science reagent supplier Biotium has developed a new labeling kit that is designed for producing antibody conjugates ideal for super-resolution stochastic optical reconstruction microscopy (STORM).
Mix-n-Stain STORM CF Dye Antibody Labeling Kits are available with Biotium’s STORM CF Dyes, which feature exceptional brightness and photochemical properties ideal for STORM imaging. In addition, the kits are optimized to yield a low degree of labeling that is optimal for single molecule localization microscopy techniques such as STORM.
STORM image quality is highly dependent on the properties of both the fluorophore and antibody conjugate. Specifically, antibodies conjugated to bright fluorophores with robust photo-switching properties at a low degree of labeling are ideal for STORM. However, a convenient method to produce antibody conjugates with a low degree of labeling for STORM is lacking. Moreover, while several red-excited dyes suitable for STORM are available, there are limited options for green-excited fluorescent probes for multi-color STORM. With the release of Mix-n-Stain STORM CF Dye Antibody Labeling Kits, Biotium has met this challenge by offering a streamlined solution for producing antibody conjugates optimal for STORM imaging in a variety of colors.
Mix-n-Stain STORM CF Dye Antibody Labeling Kits are available with 8 STORM-validated CF Dyes with emission ranging from visible red to near-IR. CF 583R and CF 597R are novel green-excited CF Dyes for multi-color STORM that were recently published in collaboration with UC Berkeley professor and STORM scientist Ke Xu, PhD.
Each Mix-n-Stain STORM CF Dye Antibody Labeling Kit is suitable for labeling exactly 50 ug antibody.
Mix-n-Stain STORM CF Dye Antibody Labeling Kit features include:
- Validated: Choice of 8 CF Dye colors developed and published for STORM
- Green-excitable options: New CF 583R and CF 597R best-in class green excited STORM dyes
- Low DOL: Labeling yields DOL of 1-2.5, optimal for STORM
- Efficient: Requires only 30-minute total reaction time with no purification after labeling
Visit the product page to learn more.
Featured image: Two-color (d)STORM imaging of a fixed COS-7 cell. Microtubules were immunolabeled with CF 647 conjugate, and mitochondria were immunolabeled with CF 583R conjugate. Photo: Bowen Wang, Michael Xiong, and Professor Ke Xu, College