Testing/Diagnostics

Coated 96-Well Plates

Available in clear, white, or black polystyrene

G-Biosciences, St Louis, offers the coated 96-well plates solution, Well-Coated™ plates. Supplied preblocked in the company’s Superior™ Blocking Buffer and available in clear, white, or black polystyrene, the plates are suitable for colorimetric, chemiluminescence, and fluorescent detection systems. The plates are offered with the following coatings: Protein A, Protein G, Protein A/G, Protein L, Goat a-Mouse antibody, or Goat a-Rabbit antibody for binding antibodies; Neutravidin, streptavidin, and biotin for biotin studies; nickel and glutathione for His- or GST-tagged recombinant proteins; and activated plates for binding proteins and other molecules through amine or sulfhydryl residues. The plates are supplied as single 96-well plates or as 12 x 8-well strips in a 96-well holder.

G-Biosciences
(314) 991-6034
www.gbiosciences.com

NMR Glass Tubes

Available in various sizes and shapes

Kimble Chase LLC, Vineland, NJ, offers Kontes brand NMR tubes. The tubes are available in various sizes and shapes including 3-, 5-, and 10-mm diameters, as round or flat-bottomed tubes, and specialty tubes equipped with valves or threaded openings. All Kontes brand NMR tubes are constructed of KIMAX® borosilicate glass with two grades: KG-33 glass is the lowest thermal expansion glass tubing offering the highest degree of thermal shock resistance for high-temperature applications, and N-51A is low thermal expansion glass tubing to accommodate ambient temperature applications. Available NMR tube closures include pressure caps comprised of polyethylene or PTFE, and red or white rubber septa.

Kimble Chase LLC
(888) 546-2531
www.kimble-chase.com

Vitamin D Assay

Measures true total 25-Hydroxy vitamin D

Diazyme Laboratories, Poway, Calif, has been given 510(k) clearance by the FDA to market its 25-Hydroxy vitamin D assay. The 25-Hydroxy vitamin D test is a homogenous assay that measures the true total 25-Hydroxy vitamin D (sum of both D3 and D2). The new assay introduces several enhancements, including a wide dynamic range with improved precision, while eliminating time-consuming washing steps and the error caused by matrix effects. It is fast and flexible. The test is user-friendly and can be used manually, or it can be easily adapted for use on a wide range of fully automated microtiter plate readers, making it suitable for use in labs of all sizes and testing needs.

Diazyme Laboratories
(888) 342-9963
www.diazyme.com

Oral Fluid Immunoassays

Available for in vitro diagnostics use

The FDA has granted clearance for six new Oral Fluid Drugs of Abuse immunoassays from Thermo Fisher Scientific Inc, Waltham, Mass. The immunoassays test for amphetamines, cannabinoids (THC), cocaine, methamphetamines, opiates, and PCP. The immunoassays are available for in vitro diagnostics use. Kits are available in a range of sizes to meet specific test volume needs. Calibrators are liquid, ready to use, and offer three-point calibration: negative, cutoff, and high. The Oral Fluid Control Set is packaged with low and high control at ±50% of cutoff and can be used with any reagent lot. Applications on a variety of chemistry analyzers are also available.

Thermo Fisher Scientific Inc
(800) 678-5599
www.thermofisher.com

Cation Column

Analysis of a variety of amines in complex sample matrices

Dionex Corp, Sunnyvale, Calif, has released the IonPac® CS19 cation-exchange column designed for gradient separation of polar and moderately hydrophobic amines plus the common inorganic cations. It is a high-capacity column allowing for the analysis of a variety of amines in complex sample matrices, including chemical process solutions, foods and beverages, and power industry samples. The column is available in 0.4- x 250-mm, 2- x 250-mm, and 4- x 250-mm formats. Key amines that can be separated using aqueous methanesulfonic acid gradients include alkanolamines, biogenic amines, methylamines, alkyl diamines, and polar amines. The CS19 column provides reproducible results. The capillary and microbore formats offer the advantage of less eluent consumption providing reduced operating costs.

Dionex Corp
(847) 295-7500
www.dionex.com

Protein Purification Kits

For the rapid purification of soluble, GST-tagged proteins

G-Biosciences, St Louis, offers HOOK™ GST Protein Purification kits for the purification of protein from bacterial samples. The kits allow for the rapid extraction of soluble, GST-tagged protein. The bacteria are first lysed with supplied Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein while maintaining the structure and activity of the protein. The GST-tagged protein is purified by affinity chromatography, and the resin is transferred to a convenient spin column where it is rapidly washed and the GST protein is eluted. The kits are available in a gravity flow format for yields of 10 mg/250 mL of culture and spin kits for rapid elution of tagged protein for ~1 mg/50 mL of culture.