PCR Biosystems, London, has launched the qPCRbio Probe 1-Step Virus Detect, a high-concentration 4x RT-qPCR kit designed specifically for ultra-sensitive, high-throughput detection of viral RNA sequences, including SARS-CoV-2. The new kit enables users to add more sample to their reactions to boost analytical sensitivity, even when working with small volume reactions.

The qPCRbio Probe 1-Step Virus Detect kit has been validated for qualitative detection of the SARS-CoV-2 nucleic acid. Validation has been performed using the Charité (Germany) primer-probe sequences targeting the RdRp and E genes as well as the Centers for Disease Control and Prevention-recommended sequences targeting specific regions of the N gene. The kit is ideally suited to high-throughput testing of covid-19 clinical samples, either with laboratory-developed assays or as a component of diagnostic testing kits.

“RT-qPCR reagent kits are pivotal for successful research and molecular diagnostic projects that aim to establish the course of viral infections,” says Alex Wilson, cofounder of PCR Biosystems. “Drawing on our PCR expertise and our commitment to delivering best-in-class technologies, we developed qPCRbio Probe 1-Step Virus Detect to offer researchers and diagnostic kit manufacturers flexibility in their assay development and ultimate confidence in the quality and accuracy of the results generated.”

qPCRbio Probe 1-Step Virus Detect has been developed with the company’s UltraScript Reverse Transcriptase enzyme, benefiting users with fast and efficient cDNA synthesis over a wide range of reaction temperatures. Reverse transcription can be performed in only 5 minutes. The kit is multiplex-compatible and can be used with all qPCR instruments, and it can also be used with a variety of probe technologies, including TaqMan, Scorpions, and molecular beacon probes.

For more information, visit PCR Biosystems.