ViaFect transfection reagent from Promega Corp, Madison, Wis, enables efficient DNA transfer into a wide variety of cell lines, including suspension cells and stem cell-derived lines. This low-toxicity reagent allows cells to stay healthy and metabolically active during transfection-based experiments—even at low cell densities—facilitating experiments where extended application of a treatment is needed. Using a three-step protocol, ViaFect transfection reagent requires minimal optimization, without removal of serum or culture medium or washing after introduction of the reagent/DNA complex. ViaFect transfection reagent contains no animal-derived components. For more information, visit Promega Corp.