The i1000 Automated Antigen Retrieval System can improve staining of monoclonal and polyclonal antibodies by overcoming the false negative staining of overfixed tissue.
It also expands the range of antibodies useful for routinely processed tissue and increases the usefulness of archival materials for retrospective studies.
Antigen retrieval is commonly performed in a microwave oven using antigen-specific retrieval solutions. Variability in results from day-to-day and lab-to-lab is not uncommon. Variables that can affect the quality, consistency and reliability of results include: microwave power; the size, color and material of the container holding the retrieval solution; the number of slides in the holder; the number of containers in the microwave; duration of retrieval; temperature of the retrieval solution; and the rate of cooling. The i1000 barcoded platform eliminates variability in antigen retrieval by controlling these variables.
A computer-controlled process maintains consistency in microwave power, temperature, duration and cooling rate. It reduces results variability due to inconsistencies in tissue processing and prevents tissue loss and damage.
The system can perform any or all of the following tasks simultaneously: dewax and antigen retrieval, dewax and H & E, and dewax. It has a throughput of 100 slides per two hours (dewax + antigen retrieval), 100 slides per hour (dewax + H & E), and 100 slides per hour (dewax). It has a loading capacity for 288 barcoded slides, automatic protocol selection and autorejection of improperly loaded slides. There are on-board protocols for more than 300 antibodies.
Keyword: system, antigen retrieval