Thermo-Start DNA Polymerase, introduced by this company, is a PCR enzyme that remains inactive until incubated at 95ºC for 15 minutes. This allows researchers to set up reactions at room temperature and permits the automation of PCR.
     The hot-start procedure inhibits non-specific annealing of primers to the template, reducing non-specific product formation. This product includes a buffer that has been designed with a hyper-thermostabilizer, which protects the enzyme from thermal damage during incubation.
     The result is increased product yields, particularly in the case of long-fragment PCR amplification. This product can be used in applications where a hot start procedure is indicated, such as multiplex PCR or PCR with small amounts of template.
Keyword: DNA, RNA, PCR