Detects HIV antigens and antibodies
Bio-Rad Laboratories Inc, Hercules, Calif, has received premarket application approval from the FDA for its fourth-generation HIV assay, the GS HIV Combo Ag/Ab EIA (enzyme immunoassay). The assay detects HIV antigens and HIV antibodies, offering early detection of HIV infections, including acute HIV infections. The GS HIV Combo Ag/Ab EIA is an enzyme immunoassay kit for the simultaneous qualitative detection of human immunodeficiency virus (HIV) p24 antigen and antibodies to HIV Type 1 (HIV-1 groups M and O) and HIV Type 2 (HIV-2) in human serum or plasma. The kit is intended to be used as an aid in the diagnosis of infection of HIV-1 and/or HIV-2 in adults and children as young as 2 years of age. The kit can run on Bio-Rad’s EVOLIS system, a self-contained automated microplate processor, and the kits may also be used with manual testing systems.
Bio-Rad Laboratories Inc
M-MLV Reverse Transcriptase
Broad thermostable activity
Bioneer Inc, Alameda, Calif, introduces RocketScript™, an M-MLV Reverse Transcriptase thermostable up to 70°C. AccuPower RocketScript RT PreMix contains Bioneer’s engineered M-MLV reverse transcriptase, RocketScript. RocketScript has broad thermostable activity (42°C~70°C), allowing efficient cDNA synthesis from virtually any RNA. The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor, and buffer components. Just add template RNA, primers, and water, and the RT reaction is ready to go.
Immobilized Monomeric Avidin Resin
Purification of biotinylated proteins
The Immobilized Monomeric Avidin Resin from G-Biosciences, St Louis, is designed for the simple purification of biotinylated proteins, antibodies, and other molecules under physiological conditions. The resin consists of monomeric subunits of avidin covalently coupled to 6% cross-linked agarose, offering a stable, reusable resin. The Immobilized Monomeric Avidin has a lower biotin-binding affinity compared to streptavidin and avidin, allowing for elution of molecules with mild elution buffers (2mM D-Biotin in 1X PBS), as opposed to routinely used strong denaturing buffers. The covalent immobilization to the agarose ensures no detectable leaching of the avidin and offers a wide tolerance to chemicals. This guarantees the resin can be reused at least 10 times with no loss of function.