Cartana, Stockholm, Sweden, has launched a new line of reagent kits designed to increase efficiency and enable in situ sequencing on virtually any tissue sample. Users will be able to analyze either fresh/fixed frozen or formalin-fixed, paraffin-embedded samples and rapidly create single-cell gene expression maps of up to 600 genes.
The reagent kits contain improved probing and sequencing technologies and come with easy-to-follow protocols for library preparation and sequencing. The high-sensitivity library preparation kit is used to probe directly up to 600 RNA targets without the need for cDNA synthesis, and generates barcoded in situ sequencing libraries with higher efficiency compared to the previous cDNA-based library preparation approach.
“Our new high-sensitivity library preparation kit has a strongly increased sensitivity, with around five to 10 times more counts than our previous cDNA-based version,” says Malte Kühnemund, vice president of research and development at Cartana.
The new kit sequences the barcodes in the libraries directly in situ, with enhanced signal-to-noise ratio and specificity compared to previous technology. The kit contains six sequencing cycles and decodes up to 600 barcodes using a standard fluorescent microscope.
For more information, visit Cartana.
Featured image: Cartana’s new line of reagent kits enable in situ sequencing on virtually any tissue sample.
