Rapid electroelution of nucleic acids and proteins
G-CAPSULE™, from G-Biosciences, St Louis, is a simple electroelution device that excises DNA or protein bands from agarose and polyacrylamide gels and elutes samples in a final volume of ~30 µL. Electroelution through the device is a gentle method that eliminates the risks of damage to DNA or protein samples commonly encountered in the use of glass milk, spin columns, binding columns, and other popular techniques. The method is simple to perform and eliminates additional steps involving washing, spinning, heating, or precipitation. The device contains two parts: G-Pick™ and G-Trap™. The user simply picks up the protein or nucleic acid band with the G-Pick and assembles it with the G-Trap. The assembled product can be submerged in electrophoresis buffer on a horizontal electrophoresis system, and the protein or nucleic acid is rapidly eluted into the G-Trap.
Detect all O-serogroups tested
ViroStat, Inc, Portland, Me, releases six new monoclonal antibodies to Salmonella. These pan-Salmonella antibodies detect all O-serogroups tested. They do not cross react with related species including E. coli, Shigella, Citrobacter, or Klebsiella, among others. The antibodies recognize the common core region of Salmonella LPS. Applications include ELISA, IFA, and lateral flow immunoassay. Pairing recommendations are also available. A downloadable data sheet for the antibodies is available on the company’s Web site.
Chromogenic culture media
HUrBi™ (HardyCHROM Urine Biplate) from Hardy Diagnostics, Santa Maria, Calif, is a chromogenic culture media that facilitates the isolation and differentiation of urinary tract pathogens. HUrBi is formulated to isolate gram-positive organisms on the left side of the biplate and gram-negative organisms on the right side of the biplate, providing total separation of organisms for easy identification. It identifies E. coli and E. faecalis with no further testing needed. Distinct color reactions for each of the common urinary tract pathogens make it easier to detect mixed infections. It is helpful in identifying gram-positive organisms such as S. aureus, S. saprophyticus, E. faecalis, and Candida spp. On the other side, it will also help identify gram-negative organisms such as Proteus spp, E. coli, Pseudomonas, and the KES Group (Klebsiella, Enterobacter, and Serratia).
For the early detection of recurrent bladder cancer
Polymedco Inc, Courtland Manor, NY, introduces the direct availability of the BTA stat®—a point of care test for the early detection of recurrent bladder cancer. The test uses monoclonal antibodies to detect the presence of bladder tumor associated antigen in urine. It is a single-step, rapid immunochromatographic assay for bladder tumor-associated antigen in voided urine. The test has a sensitivity that is considerably higher than voided urine cytology, enabling detection of recurrent early stage and grade cancers that cytology often misses. It requires only three drops of urine and delivers results in 5 minutes. The appearance of a line in the patient window indicates a positive result. The test requires one voided urine sample with no sample preparation.
Available for Dade Dimension
Stanbio Laboratory, Bourne, Tex, introduces the immediate availability of a user-defined application on the Dade Dimension® for its ß-Hydroxybutyrate assay. The assay is used for the determination of ß-hydroxybutyrate (blood ketone) in serum and plasma specimens. The measurement of ß-hydroxybutyrate is essential for the identification of complications of diabetes mellitus. The reagent set requires no reagent preparation and is designed to be used in Reagent-Start (R1/R2) systems. The liquid, ready-to-use reagents are stable until their labeled expiration dates when stored at 2°C to 8°C. The reagents are available as a set—R1: 1 x 50 mL, R2: 1 x 8.5 mL—which includes a 1.0 mmol/L standard. Controls and linearity material are available separately.
Twelve-month shelf life
OraSure Technologies, Bethlehem, Pa, introduces its new FDA-approved, CLIA-waived OraQuick ADVANCE® rapid HIV-1/2 antibody test, with a new 12-month shelf life from the date of manufacture. The rapid point-of-care test can detect antibodies to both HIV-1 and HIV-2 in 20 minutes, using oral fluid, fingerstick or venipuncture whole blood, or plasma specimens with more than 99% accuracy.