Laser Components, Olching, Germany, now offers Ultra series optical filters specifically developed for real-time quantitative polymerase chain reaction (qPCR) tests for SARS-CoV-2.
PCR is a proven method in which the inactive genetic material of viruses is exponentially multiplied so that it can be detected in the laboratory. In quantitative real-time PCR, the reaction is supplemented by the addition of fluorescent dyes that become active at a specific point during the chain reaction. The dyes makes both quantitative analysis and detection possible. The performance of this method depends heavily on how accurately the fluorescence wavelength is detected. Bandpass filters that suppress all other wavelengths ensure this.
Blocking that is greater than OD6 results in bandpass filters that have a transmission in the pass band of more than 95% and a medium wavelength tolerance of less than ± 0.3% at the wavelength needed to increase qPCR efficiency. The filter sets are optimally adapted for use with fluorescent dyes such as FAM, Cy5, HEX, JOE, ROX, TexasRed, and TAMRA.
For more information, visit Laser Components.