The 3-hydroxybutyrte (3-HB) assay test kit is for the quantitative determination of + 3-hydroxybutyrate in serum or plasma.
The primary substrates for ketone body formation are free fatty acids from adipose store. Increased hepatic ketone production and decreased peripheral tissue metabolism lead to acetoacetate accumulation in the blood. A small part is converted to acetone by spontaneous decarboxylation, but most is converted to 3-HB. Diabetes and alcohol consumption are the common causes of ketoacidosis. It has recently been shown that acetoacetate/3-hydroxybutyrate ratio in arterial blood reflects stages of liver failure.
When a sample is mixed with 3-HB R1, AcAc in the sample is broken down to acetone by acetoacetate decarboxylase (AADC). Upon addition of 3-HB R2, 3-HB in the sample is oxidized in the presence of 3-HBDH and Thio-NAD. This oxidation triggers the above cyclic reactions. Since the original AcAc in the sample has been removed, only 3-HB is assayed by measuring the rate of Thio-NADH production spectrophotometrically.
Wako
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Keywords: diabetes, alcoholism
