This L-Type HDL-C assay test kit is for the quantitative determination of high density lipoprotein cholesterol in serum. This assay is based on an immunoinhibition method where Antihuman Beta lipoprotein antibody in a first reagent binds to non-HDL lipoproteins, blocking enzyme reactions and allowing reaction only with HDL-C when the second reagent is added. Hydrogen peroxide produced by the enzyme reacts with HDL-C to yield a blue color complex upon oxidase condensation with PDAOS and 4-aminoantipyrine in the presence of peroxidase (POD). By measurement of the blue color complex produced, the HDL-C concentration of the sample can be quantitated.
This assay shows good correlation with the reference methods, no interference from coexisting substances, and is convenient with ready-to-use reagents. The benefits of this assay also include stability and a high degree of linearity. This assay can also be automated on existing clinical chemistry instrumentation.
Wako
www.wakousa.com
Keywords: cardiac marker
