Utilizing an in vitro turbidimetric methodology, the ready-to-use and sensitive Autokit assay detects albumin in human urine and can be automated using existing clinical chemistry instrumentation. The kit uses an antihuman albumin, which combines specifically with albumin in the sample. This mixture yields an insoluble aggregate. The amount of albumin is proportional to the turbidity in the sample and is measured optically at 700 nm.
The Autokit Micro albumin assay has a run precision of 1.81% CV and 0.75% CV for mean concentrations of 4.97 mg/mL and 105.81 mg/mL respectively. Linearity of the assay can extend to as much as 500 mg/mL, and the prozone phenomenon is not observed until 10,000 mg/mL.
Wako Diagnostics
(877) 714-1924; www.wakousa.com
