| The AccuClot Protein S kit is for the quantitative determination of Protein S activity in citrated human plasma using a clotting assay. Protein S, a Vitamin K-dependent plasma protein, serves as a cofactor for the anticoagulant activity of Protein C in the degradation of Factors V and VII. This assay is similar to a standard Intrinsic Factor clotting assay. Dilutions of normal plasma are mixed with Protein S depleted plasma. The mixed plasma is activated by a reagent that contains Factor Xa, activated protein C and phospholipid. After activation, clot formation is initiated with the addition of calcium chloride. Under these conditions, the clotting time is directly proportional to the Protein S concentration. The Factor Xa activator minimizes potential interference for Factor V Leiden mutation and high levels of Factor VIII. The product is heparin neutralizer (up to 1.2µ/mL), allowing Protein S analysis on heparin-therapy patients. Sigma Diagnostics keyword: co-ag, reagent |
