Pancreatic lipase in serum is associated with pancreatic diseases. Pancreatic lipase measurement has been reported using titrimetric, turbidimetric, fluorometric and colorimetric methodologies.
The Pancreatic Lipase Color Assay uses a clear substrate solution of 1, 2-diglyceride, which is a natural substrate. The assay is highly sensitive and specific for pancreatic lipase, using colipase and deoxycholate as activators. The assay shows reproducibility and stability, and the simplicity of the procedure makes it adaptable for use on automatic analyzers.
Serum lipase acts on a natural substrate, 1, 2-diglyceride, to liberate 2-monoglyceride. This is hydrolyzed by monoglyceride lipase, a highly specific enzyme for monoglyceride, into glycerol and free fatty acid. Glycerol kinase acts on glycerol to form glycerol-3-phosphate, which is in turn acted on by glycerol-3-phosphate oxidase to generate hydrogen peroxide. Peroxidase converts the hydrogen peroxide, 4-aminoantipyrine and TOOS (N-ethyl-N-(2-hydroxy-3-sulfopropyl-)-m-toluidine) into a quinone dye.
The rate of formation of the dye, measured as an increase in absorbance at 550nm, is proportional to the lipase concentration in the sample. This product is available in a variety of kit sizes from 5mL to three liters. Applications are available for a number of automated chemistry analyzers. Equal Diagnostics