The cfPure Cell Free DNA Extraction Kit from Amsbio is a magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma or serum samples.
Using a quick, simple, and automation-friendly protocol, the kit allows users to quickly extract high-quality cfDNA that is ready for use in downstream applications including next-generation sequencing, Bisulfite Sequencing, PCR, qPCR, ddPCR, and other demanding applications. This optimized protocol gives highly reproducible results and higher yields relative to other magnetic bead-based methods.
Using silica-coated paramagnetic particles, cfPure kits were developed to ensure efficient recovery of 100bp-500bp DNA fragments in order to maximize recovery of low molecular weight cell-free DNA. Designed for scalability (100 µl to 10 mL of serum or plasma), cfPure kits enable the use of just the number of beads and amount of reagent needed for a particular cfDNA extraction.
In addition to being simple to use, scalable, and easy to automate, cfPure kits allow labs to use less magnetic bead solution when purifying small amounts of plasma, which saves money. Since cfDNA is typically found at very low concentrations and in a fragmented state, very efficient DNA capture, as provided by cfPure Cell Free DNA Extraction Kits, is vital to provide sensitive and reliable results.
For further information, visit Amsbio.
Featured image: Circulating cell free DNA. (Courtesy: Amsbio)