This L-Type UIBC reagent kit, for quantitating unsaturated iron binding capacity, is based on a method that uses BPT as a chromogen.
When a sample is mixed with buffer containing a known excess amount of iron, unsaturated transferrin in serum quantitatively associates with iron in the buffer and is converted to a saturated state. The portion of iron that remains unassociated is assayed through color development with ascorbic acid and BPT disulfonic acid disodium salt.
The unsaturated transferrin concentration in the sample can be determined by calculating the decreased iron in the buffer. Concentrations are measured spectrophotometrically at 545nm.
This liquid, ready-to-use reagent is highly specific. It has no significant interference by ascorbic acid, bilirubin, hemoglobin or intrafat. The within-run precision is 0.65 percent CV and 0.85 percent CV at mean concentrations of 183.6µg/dL and 102.3µg/dL, respectively. The total precision is 5.28 percent CV and 2.95 percent CV at mean concentrations of 115.8µg/dL and 187.6µg/dL, respectively. The minimum detectable limit is 2µg/dL with a linearity limit of 600µg/dL.
Wako Diagnostics
www.wakousa.com
Keyword: assay, iron binding
