A new set of molecular biomarkers in blood plasma have been identified that could lead to the development of improved diagnostic tests and screening for Alzheimer’s disease, according to research from the Mayo Clinic.
The Mayo Clinic study, published in eBioMedicine, is a study that focused on RNA molecules in plasma as biomarkers to detect Alzheimer’s disease in African Americans — the population at greatest risk for developing Alzheimer’s disease. This approach enabled researchers to pinpoint specific molecules in plasma that could serve as biomarkers to confirm a diagnosis of Alzheimer’s disease in this population.
The study builds on previous research that identified genetic risk factors for Alzheimer’s disease and established that RNA molecules in blood plasma could potentially be used as biomarkers.
In the study, researchers examined blood plasma messenger RNA molecules in 151 African Americans diagnosed with Alzheimer’s disease and 269 African Americans diagnosed as cognitively unimpaired with Clinical Dementia Rating scale scores of zero.
The researchers found that when the plasma levels of six messenger RNA molecules — encoded by genes CLU, APP, CD14, ABCA7, AKAP9 and APOE — were accounted for in their statistical models, they improved their ability to accurately identify participants with an Alzheimer’s diagnosis by 8%. Researchers explain this is an improvement, compared to statistical models that account for only the presence of known risk factors, such as age and sex, and whether the person is a carrier of the APOE-e4 allele — a gene known to increase the risk of Alzheimer’s
The researchers say this discovery could lead to more accurate Alzheimer’s disease screening for everyone, particularly for the people and communities at greatest risk.
“Having a comprehensive panel of biomarkers for use in screening will help with early detection of Alzheimer’s disease, and it will also contribute to intervention strategies that can delay and mitigate the onset of the disease,” says Joseph Reddy, PhD, a Mayo Clinic quantitative health sciences researcher and first author. “This could be especially relevant for African Americans — a population underrepresented in Alzheimer’s disease research — who were the focus of this study.”
The researchers predict that this discovery could contribute to the development of more accessible, minimally invasive screening options, enabling improved disease management.
“Many screening tests for Alzheimer’s disease may not be accessible to all patients due to cost or lack of availability at health care facilities in their area,” says Minerva Carrasquillo, PhD, a Mayo Clinic neurogeneticist and senior author. “Some tests rely on complex imaging techniques, or on obtaining a sample of cerebrospinal fluid from the patient. Obtaining a plasma sample only requires a blood draw, which is a routine procedure in most clinical settings.”
The researchers indicate that future research will focus on identifying additional genetic biomarkers in blood plasma that may improve the accuracy of Alzheimer’s disease diagnostic tests.
This research was funded by grants from the National Institute on Aging and the Florida Department of Health Ed and Ethel Moore Alzheimer’s Disease Research Program. This research also was supported by the Mayo Clinic’s Memory Disorders Center, Alzheimer’s Disease Research Center, and Center for Health Equity and Community Engagement Research.
The researchers declare no conflicts of interest.