The Detector RNA in vitro Transcription Biotinylation Kit provides an efficient method for biotinylating single-stranded RNA probes during in vitro transcription.
The resulting probes are very hot and can exhibit higher stability and sensitivity than DNA probes. Both SP6 and T7 RNA polymerase promoters are included for flexibility in transcript initiation. The b-actin positive-control template can be used as a control probe in hybridization assays. Kirkegaard & Perry Laboratories